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. 2018 May 14;7:e32919. doi: 10.7554/eLife.32919

Figure 6. PI3Kα signals are required for macrophage RAE-1δ induction by CSF-1.

(A) Peritoneal wash cells were stimulated with CSF-1 plus vehicle control or PI3Kα inhibitors at 3 μM, and macrophage RAE-1δ was analyzed at 24 hr. (B). Relative macrophage RAE-1δ MFI 24 hr after stimulation with CSF-1 plus the indicated concentrations of the indicated PI3K inhibitors. (C) Relative Raet1d mRNA levels 24 hr after macrophage stimulation with CSF-1 plus vehicle control or PI3Kα inhibitors at 3 μM. Statistical significance was determined using one-way ANOVA with Bonferroni post-tests. Data are representative of 3–4 independent experiments.

Figure 6.

Figure 6—figure supplement 1. Induction of phospho-S6 by CSF-1.

Figure 6—figure supplement 1.

(A) Phospho-S6 staining (top) and MFI quantification (bottom) in macrophages stimulated with control medium or 10 ng/ml CSF-1 for the indicated times. Data are representative of 3 independent experiments.