Figure 3. MG-derived GFP⁺ cells exhibit larger and faster visual responses than normal GFP⁻ MG.

a–c, Example recordings from GFP⁻ MG (a), MG-derived GFP⁺ cell (b) and GFP⁻ cone bipolar cell (c). Traces are visual responses to 500-ms steps in luminance, recorded in current-clamp. d, Population data for input resistance and resting membrane potential measurements. e, Population data for visual responses recorded with the current-clamp technique. f, Population data for light-evoked excitatory and inhibitory synaptic inputs recorded with the voltage-clamp technique. All cells recorded were from five ANT-treated mice. Recordings were performed at 4, 5, 10, 12 and 15 weeks post-TSA. Visual responses were found in cells regardless of post-TSA duration. GFP⁻ MG: V_rest, −81 ± 6 mV; R_in,41 ±17 MΩ; maximal response to light stimulation, 0.95 ± 0.52 mV; n = 3. GFP⁺ cells: V_rest, −46±11 mV, n = 19; R_in, 871 ±868 MΩ, n = 20; maximal response to light stimulation, 3.2 ±2.7 mV, n = 8; excitatory input, −10±8 pA, n = 8; inhibitory input, 10 ±7 pA, n = 4. GFP⁻ neurons: V_rest, −42±6 mV, n = 10; R_in, 1,088 ±836 MΩ, n = 10; maximal response to light stimulation, 13 ±6 mV, n = 4. All responses are written mean ±s.d.