Extended Data Figure 1. Animal age and numbers used in study.
a, Histogram showing ages at which all Glast-creER and Rlbp1-creER mice used for the study received their first tamoxifen injection. There were 108 Glast-creER and Rlbp1-creER mice used for the study (122.7 ± 64.1 days, mean postnatal age ±s.d.). b, Histogram showing ages at which all ANT-treated mice used for the study received their first tamoxifen injection. There were 54 ANT-treated mice used for the study (136.4 ± 57.8 days, mean postnatal age ± s.d.). ANT-treated mice are classified according to experimental assay (H3K27ac ChIP-PCR; RNA-seq (10x Genomics)/H3K27ac ChIP-PCR, cells from these mice were split for both assays; RNA-seq (Fluidigm), single-cell RNA-seq; Otx2 ChIP–seq; IHC, immunohistochemistry; SBFSEM, serial block-face scanning electron microscopy; Ephys, whole-cell electrophysiology; ATAC–seq).