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. Author manuscript; available in PMC: 2018 Aug 3.
Published in final edited form as: Nature. 2017 Jul 26;548(7665):103–107. doi: 10.1038/nature23283

Extended Data Figure 4. MG-derived neurons form synaptic specializations in IPL.

Extended Data Figure 4

a, Confocal images of MG-derived bipolar-like cells with processes in IPL (scale bars, 10 μm). b, Super-resolution Zeiss Airyscan raw images of white boxes from a showing IPL processes stained with presynaptic ribbon marker CtBP2 (Ribeye, white) and postsynaptic marker PSD95 (magenta). c, Images from b masked in Amira to show presynaptic CtBP2 within GFP processes. d, Images of masked presynaptic CtBP2 staining apposed to postsynaptic PSD95 staining. e, Images of masks that were traced in Amira around GFP processes in IPL (scale bars, 10 μm). f, Images of masked CtBP2 staining that is within GFP processes. g, Images of CtBP2 superposed with PSD95 staining from c (white arrows) rotated in xz plane (scale bars, 1 μm). Images are from retinas that were collected 11 days post-TSA.

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