FIG 5.

Phenotype of Ppp1r3b-KO mice (A) and mice infected with Ad-mouse PPPR1R3B. (A) Food intake was synchronized for 3 days (18 h fasting, 6 h feeding) in male mice (8 wk old, n=3-5/group). At the end of the fasting cycle on the 4th day, the mice were killed 4 h after they were fed. The experiment was repeated and the results were similar. P-values by t-test. * = P<0.05; ** = P<0.001 (B) Phenotype of mice overexpressing mouse PPP1R3B. The tail veins of 8-week old male mice (10/group) were injected with adenovirus expressing PPP1R3B (Ad-PPP1R3B) or an empty control virus (Ad-RR5) (1.25×10¹¹ viral particles per mouse). The chow-fed mice were sacrificed 48 h after the injection. P-values by t-test. * = P<0.05; ** = P<0.01; ***P<0.001’ ****P<0.0001.