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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Hepatology. 2018 Jun;67(6):2384–2396. doi: 10.1002/hep.29733

Fig. 5. Chi3l1 production is dependent on IFN-γ and TNF-α.

Fig. 5

(A) Con A-induced up-regulation of IFN-γ, TNF-α, and Chi3l1 mRNA levels in the liver were measured in WT mice at various time points (n=4 mice per group). (B–F) WT mice were treated with anti-IFN-γ and TNF-α antibodies by i.p. injection at the same time of Con A administration and sacrificed at 3h post-Con A treatment. The hepatic mRNA levels of Chi3l1 (B), and protein levels of Chi3l1 in the liver (C) and serum (D) were measured (n=3–5 mice per group). (E–F) LSEC and MΦ were isolated from naïve WT mice and treated with IFN-γ (50ng/ml) or TNF-α (50ng/ml) for 3h in vitro. Chi3l1 mRNA expression levels were measured by qRT-PCR. P values are as indicated. One-way ANOVA was performed in A–F.