(A–D) Male Chi3l1−/− mice were treated with Con A as described in Fig. 1 or recombinant TF(rTF), anti-TF (1H1) antibody or rChi3l1 as indicated. (A, C) Immunohistochemical staining for fibrin(ogen) (magnification: 200x), and H&E-stained liver sections (magnification, 100×) were performed at 24h after Con A treatment. Necrotic areas are outlined. (B, D) Serum ALT levels were determined at 24 h post-Con A treatment (n=5 mice per group). Images shown are representative of at least 4 mice per group. P values are as indicated. One-way ANOVA in A, C.