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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Eur J Immunol. 2018 Feb 22;48(5):791–802. doi: 10.1002/eji.201747240

Figure 4. Reconstitution of fH+/− mice with recombinant factor H does not protect them from kidney injury after ischemia/reperfusion.

Figure 4

fH+/− mice were injected intra-peritoneally with recombinant factor H (fH) or with PBS as a vehicle control and subjected to ischemia/reperfusion of the kidneys. A) The purity and identify of the ~150 kD fH was confirmed by Coomassie staining (Coom) and Western blot analysis. B) After 24 hours the serum urea nitrogen (SUN) levels were measured using a NADH oxidation assay. Values in the two groups of mice were not significantly different by unpaired student t test. C) After 24 hours tissues were collected, and kidney sections from the two mice injected with fluorescently tagged factor H were stained for C3d and imaged by confocal microscopy. Original magnification x600 and scale bar = 50 μm. Data shown is representative of four mice that were analyzed from two independent experiments.