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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Eur J Immunol. 2018 Feb 22;48(5):791–802. doi: 10.1002/eji.201747240

Figure 9. Monoclonal IgM binds to glomerular epitopes in factor H deficient mice after ischemia/reperfusion but does not cause C3 deposition.

Figure 9

Mice deficient in factor H and soluble IgM deficient mice (fH+/−sIgM−/− mice) were injected intravenously with purified C2 IgM or with an equal volume of a vehicle control (Veh), and the mice were then subjected to ischemia/reperfusion of the kidneys. After 24 hours, the mice were sacrificed. A) The serum urea nitrogen (SUN) and serum creatinine levels in Veh and C2 injected mice were measured using a NADH oxidation assay and a picric acid chromogenic assay, respectively. No significant difference was observed (unpaired student t test). B) Tissue sections were stained with antibodies to murine C3 and IgM. Immunofluorescence microcopy for IgM and C3. Original magnification x600. Scale bar = 50 μm. Data shown in A is pooled from one experiment. Each data point represents one mouse, and the bar represents the group mean. Images in B are representative of six mice for each group from two independent experiments.