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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Stem Cells. 2018 Feb 25;36(6):868–880. doi: 10.1002/stem.2793

Figure 3. Elongation of telomeres partially restores youthful cell fate in CPCs.

Figure 3

A. Immunoblot and densitometric analyses of endogenous telomerase reverse transcriptase (TERT) and green fluorescent protein (GFP) in CPCs transfected with 0.6–2μg/ml modified mRNA expressing GFP or wild type TERT (TERT-WT) (N=8). GFP expression indicates success of transfection. B. Mean fluorescence intensity of telomere staining as determined by qFISH (N=39–56 cells from 3 experiments). C. Cell counts as determined by microscopic counting of live cells at 4 days after plating (N=4). D. Senescent cell counts as determined after staining with SA-β-gal (N=3). E. Immunoblots and densitometric analyses of p27, Tie2 and p57 expression (N=4–5). For all experiments, except D, data is expressed relative to GFP transfected CPCs of each strain. *p<0.05, **p<0.01, ***p<0.001. Multiple t-tests (A–E).