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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Stem Cells. 2018 Feb 27;36(6):943–950. doi: 10.1002/stem.2800

Figure 4. The anti-apoptotic effect of Nfix in HSPC depends on c-MPL signaling.

Figure 4

(A) Relative number of cells in control and NFIX+ cultures (i) with or without TPO and (ii) with or without AMM2 72 hours after replating (TPO, n = 3; AMM2, n = 6). Bar indicates significant difference in the extent of cell loss after 72 hours of culture between control and NFIX+ cells. (B) Percentage of GFP+ cells in control and NFIX+ cultures (i) with or without TPO and (ii) with or without AMM2 72 hours after replating (TPO, n = 3; AMM2, n = 6). (C) Representative fluorescence histograms of GFP+ control and NFIX+ cultures to illustrate shift in c-KIT intensity 72 hours after removal of mTPO or addition of AMM2 (TPO, n = 3; AMM2, n = 6). (D) Relative levels of apoptosis in GFP+ compartment of control and NFIX+ cultures (i) with or without TPO and (ii) with or without AMM2 72 hours after replating (TPO, n = 3; AMM2, n = 6). All values represent mean ± standard deviation. NS denotes not significant.