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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: FEBS J. 2018 Apr 16;285(10):1827–1839. doi: 10.1111/febs.14449

Figure 3.

Figure 3

In vitro fertilization (IVF) with cumulus intact (CC+) and zona pellucida-free (ZP−) oocytes. (A) CDO−/− sperm had lower fertilizing ability compared with that of WT sperm using either CC+ or ZP− oocytes (*P < 0.05). Data are means ± SE of five independent replicates for each experiment. (B) Acrosome exocytosis (AE) of WT and CDO−/− sperm co-incubated with CC+ for 6 h were not significantly different. Data are means ± SE from four independent replicates. (C) Tyrosine phosphorylation of WT and CDO−/− sperm proteins after incubation under non-capacitating (NC) or capacitating conditions (CAP). No difference was observed in the capacitation-induced phosphorylation profile of WT and CDO−/− sperm. A representative immunoblot is shown from three replicate experiments. (D) Hyperactivation of WT and CDO−/− sperm incubated under CAP condition. No difference was observed between genotypes. Data are means ± SE from four independent replicates.