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. Author manuscript; available in PMC: 2018 Nov 30.
Published in final edited form as: Nature. 2018 May 30;558(7708):136–140. doi: 10.1038/s41586-018-0162-7

Figure 4. Expression of beclin 1 F121A prevents lethality of Klotho-deficient mice.

Figure 4

a, Co-immunoprecipitation of beclin 1 and Bcl-2 in kidneys from mice of indicated genotype (n = 3 animals per genotype). b, Quantitation of beclin 1 co-immunoprecipitated with Bcl-2 in (a). c, Western blot analysis of autophagy markers in kidney from mice of indicated genotype. d, Quantitation of western blot analysis of p62 levels and LC3-II/I ratios (combined results are for six mice per genotype; each independent experiment had 1 mouse per genotype). Bars represent mean ± s.e.m. e–g, Kaplan-Meier survival curves for mice of indicated genotype, showing the lifespan of all mice in cohort (e), females alone (f) or males alone (g). N = number of mice per group. h, Representative images of 10 week-old mice of indicated genotype for body size comparison. Scale bars, 1 cm. i, Serial body weights in female and male mice of indicated genotype. Data points and error bars represent mean ± s.e.m for 12 mice per genotype. P-values in (b) and (d), one-sided unpaired t-test. P-values in (e–g), log-rank (Mantel-Cox) test. WCL, whole cell lysate. WT, wild-type mice; beclin 1 KI, Becn1F121A/F121A mice; Klotho HM, klothoHM/HM mice and beclin 1 KI/Klotho HM, Becn1F121A/F121A; klothoHM/HM mice. For uncropped gels, see Supplementary Fig. 1.