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. Author manuscript; available in PMC: 2018 Jun 8.
Published in final edited form as: Biochemistry. 2016 Nov 4;57(29):4312–4324. doi: 10.1021/acs.biochem.6b00964

Figure 6.

Figure 6

pH sensitivity of Dnmt3b-C processive catalysis. (A) Methylation at one- and two-site substrates by Dnmt3b-C was measured for 1 h at pH 6.5 or 7.5. (B) Ratio of the methylation levels calculated as the fold change at two-site vs one-site substrate. (C and D) Time course of DNA methylation by Dnmt3b-C at pH 6.5 for one- and two-site substrates. For each substrate, the time course of DNA methylation reaction was determined in the presence of 0.5–2 μM Dnmt3b-C. The reaction was started by the addition of enzyme, and the data were fitted by linear regression, which was weighted by 1/Y2. (E) The calculated methylation rates at pH 6.5 were plotted vs enzyme concentration and compared to those at pH 7.5. Data are the average ± the standard error of the mean (n ≥ 4 independent experiments).