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. 2018 May 31;102(6):1158–1168. doi: 10.1016/j.ajhg.2018.04.012

Figure 4.

Figure 4

βIV Spectrin Protein and Lipid Interactions

(A) Cotransfections and immunoprecipitations between AnkG-GFP and the indicated βIV spectrin constructs. Immunoprecipitation reactions were performed with anti-GFP antibodies, and immunoblots were performed wih anti-Myc antibodies. Both input and immunoprecipitates (IPs) are shown.

(B) Cotransfection and immunoprecipitation of αII spectrin and βIV spectrin’s N terminus, including spectrin repeat 2 with and without the p.Arg504Gln variant. IPs were performed with anti-GFP antibodies, and immunoblots were performed with anti-Myc antibodies.

(C) Immunostaining of HEK cells transfected with the indicated βIV spectrin PH domain constructs. The scale bar represents 10 μm.

(D) Immunoblot of GST and βIV spectrin PH-domain-containing fusion proteins and PIP strip blots showing that the same fusion proteins bind to phosphoinositides. GST fusion proteins were detected with anti-GST antibodies.