Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 7;8:8733. doi: 10.1038/s41598-018-24956-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Validation of sgRNAs specific binding to the endogenous Myc promoter in CRISPR activation system. (a) Primers flanking the sgRNA-M5 binding motif was used for ChIP-PCR in Trp53-null neurosphere cells infected with Lenti-dCas9-VP64-T2A-GFP and Lenti-U6-sgRNA-RFP with sgRNA-M5 or combination with sgRNA-M5, 7, and 9. sgRNA-M12 was used as a negative control. Full agarose gel is presented in Supplementary Fig. S6. (b) Enrichment of sgRNA-M5, M7 and M9 binding to pre-designated loci in Trp53-null neurosphere cells revealed by ChIP-Seq using dCas9 antibody. ChIP-Seq of H3K27ac on mouse G3 MB cell lines MB1 and MB3 illustrated to define open chromatin status of the endogenous Myc locus. (c) Mapping reads from ChIP-Seq of dCas9 used to characterize on-target (M5, M9) and off-target (M7) sequences.