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. 2018 Jun 7;9(6):692. doi: 10.1038/s41419-018-0727-2

Fig. 1. Generation and genotyping of cardiac-specific Ctsk-CKO mice.

Fig. 1

a tm1a allele: representing targeted Ctsk allele containing LacZ reporter-promoter-driven neo targeting cassette, FLP-FRT sites, Cre-loxP sites and Ctsk exons (numbered 1–8); b tm1c allele: the conditional allele representing the “floxed” mice generated by crossing “tm1a” mice to FLP recombinase transgenic mice; c tm1d allele: the null allele representing the cardiac-specific Ctsk-conditional knockout mice generated by crossing “tm1c” (floxed) mice to Myh-Cre recombinase transgenic mice. The FRT sites are indicated by green triangles, the loxP sites are represented by red triangles, the exons are represented by orange rectangles. d Representative image of LacZ genotyping: LacZ positive targeted band is 77 bp; LacZ negative mice display no bands. e Representative image of FLP genotyping: mutant-band is 340 bp; wild-type band is 650 bp; heterozygotes have both bands; homozygotes only have bands at 340 bp; mice have mutant bands are FLP positive. f Representative image of Myh-Cre genotyping: mutant-band is 300 bp; internal control band is 200 bp; mice have mutant bands are Myh-Cre positive. Both Myh-Cre positive and FLP positive are cardiac-specific Ctsk-CKO mice. Mice 1, 2, 4, 5, and 6 are used as cardiac-specific Ctsk-CKO (Myh-Cre+; Ctskfl/fl); mice 9 ~ 10 are used as control (Myh-Cre−; Ctskfl/fl)