Table 2.

Assay performance characteristics showing PCR efficiency E, linear dynamic range (LDR), slope, precision and associated correlation coefficient R² (see Materials and Methods).

Gene	E (%)	Slope	LDR (ng)	Precision	R2
Actb	95	−3.45	0.07–700	0.18	0.999
Atp5f1	104	−3.23	0.07–70	0.31	0.999
B2m	100	−3.31	0.07–70	0.22	0.999
Gapdh	96	−3.42	0.07–70	0.29	0.998
Gusb	163	−2.39	7–700	0.38	0.991
Hprt	143	−2.60	7–700	0.12	0.995
Pgk1	101	−3.29	0.07–700	0.26	1.0
Ppia	100	−3.32	0.07–70	0.15	0.997
Rps18	99	−3.34	0.07–700	0.16	0.998
Tbp	106	−3.18	0.7–700	0.21	0.997
Tfrc	114	−3.02	0.07–700	0.36	0.999
Ywhaz	102	−3.27	0.7–700	0.17	0.999