Table 3.
Ranking of the candidate reference genes according to five different evaluation methods.
| Gene | Comprehensive Ranking | Delta Ct | geNorm | NormFinder | BestKeeper | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Value | Rank | SD aver. | Rank | M value | Rank | Stability | Rank | SD | Rank | |
| Atp5f1 | 1.00 | 1 | 1.03 | 1 | 0.362 | 1 | 0.288 | 1 | 0.40 | 1 |
| Pgk1 | 1.86 | 2 | 1.12 | 3 | 0.362 | 1 | 0.489 | 2 | 0.46 | 2 |
| Gapdh | 2.91 | 3 | 1.11 | 2 | 0.504 | 2 | 0.516 | 4 | 0.48 | 3 |
| Tfrc | 4.23 | 4 | 1.14 | 4 | 0.560 | 3 | 0.640 | 5 | 0.52 | 4 |
| Ppia | 6.12 | 7 | 1.18 | 5 | 0.580 | 4 | 0.742 | 7 | 0.67 | 8 |
| Gusb | 5.66 | 5 | 1.22 | 7 | 0.729 | 6 | 0.502 | 3 | 0.60 | 7 |
| Ywhaz | 6.00 | 6 | 1.21 | 6 | 0.643 | 5 | 0.716 | 6 | 0.55 | 6 |
| B2m | 7.11 | 8 | 1.31 | 8 | 0.790 | 7 | 0.784 | 8 | 0.54 | 5 |
| Actb | 9.24 | 9 | 1.45 | 9 | 0.847 | 8 | 1.140 | 9 | 0.89 | 10 |
| Tbp | 9.74 | 10 | 1.59 | 10 | 0.952 | 9 | 1.194 | 10 | 0.86 | 9 |
| Hprt | 11.00 | 11 | 2.07 | 11 | 1.154 | 10 | 1.767 | 11 | 1.35 | 11 |
| Rps18 | 12.00 | 12 | 2.88 | 12 | 1.441 | 11 | 2.750 | 12 | 2.11 | 12 |
Atp5f1, Pgk1 and Gapdh were ranked as the most stable candidate reference genes, while Rps18, Hprt, and Tbp/Actb were designated as the least stable ones.