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. 2018 Jun 7;8:8706. doi: 10.1038/s41598-018-26818-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of the L697W mutation on MYO3A function in the presence of ESPN-1. (A) Confocal images of transfected COS7 cells showing filopodia tip localization of GFP-MYO3A WT (green) or (B) GFP-MYO3A L697W (green) in presence of mCherry-ESPN-1 (red). (C,D) Filopodia length and initiation activity in WT and m-Cherry-ESPN-1 (WT + ESPN-1), and L697W and m-Cherry-ESPN-1 (L697W + ESPN-1) coexpressing cells are shown and represented as in Fig. 4. (E) Filopodia tip localization of WT (green) or L697W (F) when coexpressed with mCherry-MYO3A WT (red) and untagged ESPN-1 in transfected COS7 cells. (G,H) Filopodia length and initiation activity of transfected COS7 cells coexpressing WT, mCherry-MYO3A WT and untagged ESPN-1 (WT + WT + ESPN-1) and COS7 cells coexpressing L697W, mCherry-MYO3A WT and untagged ESPN-1 (L697W + WT + ESPN-1) represented as in Fig. 4. For all the confocal images, F-actin was stained with AlexaFluor®-405 phalloidin and it is shown in blue.