Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 7;8:8706. doi: 10.1038/s41598-018-26818-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Cargo specific tip localization behavior of MYO3A L697W. (A) Close-up view of representative filopodia and fluorescence profiles along the filopodia length of COS7 cells coexpressing wild-type GFP-MYO3A WT (green) and mCherry-MYO3A WT (red). (B) Expression pattern and fluorescence profiles of mutant GFP-MYO3A L697W (green) and mCherry-MYO3A WT (red) proteins in a representative COS7 cell filopodia (C) Filopodia localization and fluorescence profiles of GFP-MYO3A WT (green), mCherry-MYO3A WT (red) proteins in a COS7 cell coexpressing GFP-MYO3A WT, mCherry-MYO3A WT and untagged ESPN-1. (D) Close-up view of filopodia and fluorescence profiles along it of COS7 cells coexpressing GFP-MYO3A L697W (green), mCherry-MYO3A WT (red), actin (in blue) and untagged ESPN-1. The sum of the green and red channels (black trace in the fluoresce profile) shows the same profile as MYO3A WT in the previous panel. (E–G) Confocal images of transfected mouse auditory (E) and vestibular (G) hair cells coexpressing mCherry-MYO3A WT (red) and GFP-MYO3A L697W (green) proteins. (F–H) Fluorescence profiles along the stereocilia length of auditory (F) and vestibular (H) hair cells coexpressing mCherry-MYO3AWT (red) and GFP-MYO3A L697W (green). For all the images, actin was stained with AlexaFluor®-405 phalloidin and it is shown in blue.