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. 2018 Jun 7;8:8704. doi: 10.1038/s41598-018-27021-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HGSOC cells can be characterized by mesenchymal/epithelial status. (A) mRNA expression levels of SNAI1 (Snail; black), CDH1 (E-cad; white), and CDH2 (N-cad; gray bars) in indicated lines relative to fibroblasts by qRT-PCR. β-actin was used for normalization. (B) RNA S/E index: qRT-PCR data from A were combined to describe the ratio between SNAI1 and CDH1 mRNA. (C) Protein levels of Snail and E-cad from Western blot (WB), normalized to α/β tubulin, relative to NCCIT. For each experiment, all samples were run on a single gel. Gels from independent experiments were analysed in parallel. (D) Protein S/E index: WB data from C were combined to describe the ratio between Snail and E-cad. (A–D) Data represent the mean of at least three independent experiments. Error bars, SEM. (E) DIC images of cell lines.