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. 2018 Jun 7;8:8702. doi: 10.1038/s41598-018-27027-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of differentially expressed genes and predicted upstream regulators by qRT-PCR. hSeC were infected with ZIKV (PRVABC59 at MOI 1) and qRT-PCR was conducted on RNA extracted to validate (A) type I interferons and ISGs IFIT1 and MXA at 24 and 72 hpi, and RNA virus-sensing PRRs TLR3, DHX58 (LGP2), DDX58 (RIG-I), and IFIH1 (MDA5) at 24 and 72 hpi. (B) Validation and comparison of ISG IFIT1 and cytokines IL6, and IFNB (IFN-β) and TNFα gene expression in hSeC at 72 hpi infected with MR766 and PRVABC59 (MOI 1). Fold-change as compared to mock was calculated by normalizing the data to GAPDH and data represents mean ± SD from at least three independent experiments.