Fig. 1.
mtDNA dysfunction affects CII assembly. a NBGE analyses of CII assembly from digitonin-solubilized mitochondria isolated from 4T1 and MCF7 cells and their ρ0 counterparts. b NBGE showing formation of CIIlow and depletion of CIII assembly upon suppression of expression of mtDNA-encoded genes with CAB at indicated time points. c, d NBGE of CII using mitochondria isolated from 4T1 cells transfected with siRNA against SDHA, SDHB, or SDHC. e WB after SDS-PAGE of steady-state levels of CII subunits in 4T1 cells treated with siRNAs as shown (left panel). Right panel shows quantification of WB in the left panel related to actin. The numbers ‘1’ and ‘2’ in c–e refer to two different siRNAs. Data shown are mean values ± SD; images are representative of three independent experiments