Fig. 2.

SDHB depletion stabilizes CII_low. a A scheme showing the structure of the SDHB gene and exon1 as the TALEN target. b DNA sequence alignment of the SDHB exon 1 from the GenBank nucleotide sequence database, and parental and SDHB^KO MDA231 cells. Sanger sequencing was done from the PCR product amplified using two pairs of primers (PR1 and PR2) using genomic DNA as a template. c WB after SDS-PAGE of CII subunits in individual sublines, as shown. SDHB^KOEV cells are SDHB^KO cells transfected with empty vector and were used as a control for stable shRNA transfections. d NBGE of mitochondria isolated from parental, SDHB^KO, SDHB^KOEV, and SDHB^KOSDHA^low cells showing three variants of SDHA, using anti-SDHA IgG and anti-SDHB IgG. e Proliferation of MDA231 subline was analyzed at the indicated time points using the crystal violet method. Panel on the right shows crystal violet staining at 1, 4, and 7 days. Data are normalized to day 1. Data shown are mean values ± SD (n = 3); images are representative of three independent experiments