Fig. 8.

CII assembly and de novo pyrimidine pathway in paragangliomas. a Histological examination of SDHA and SDHB expression from tumor tissue of paraganglioma patients with SDHA or SDHB mutations or with sporadic paragangliomas. Tissues were processed for H&E staining and immunohistochemistry using SDHA and SDHB IgGs. Representative images are collected using a 40× objective. Scale bars represent 100 μm. b–d Paraganglioma tissues, as shown, were assessed by WB following protein separation by NBGE for CII assembly using anti-SDHA IgG (b), and by SDS-PAGE for CAD and FAD (c) and for markers of cell cycle (d). Images are representative of three independent experiments