Table 6.
PCR primers for Ancylostoma spp. and Giardia.
| Target | Primer name | Sequence | Reference |
|---|---|---|---|
| Ancylostoma spp . | RTHWIF | 5′-GATGAGCATTGCWTGAATGCCG-3′ | 50 |
| RTHWIR | 5′-GCAAGTRCCGTTCGACAAACAG-3′ | ||
| Giardia gdh | |||
| Conventional PCR | Primary reaction | 52, 53 | |
| GDHeF | 5′TCAACGTYAAYCGYGGYTTCCGT-3′ | ||
| GDH 2 | 5′-ACCTCGTTCTGRGTGGCGCA-3′ | ||
| Secondary reaction | |||
| GDHiF | 5′-CAGTACAACTCYGCTCTCGG-3′ | ||
| GDH 4 | 5′-GTGGCGCARGGCATGATGCA-3′ | ||
| qPCR | gdhF1 | 5′-GGGCAAGTCCGACAACGA -3′ | 51 |
| gdhR1 | 5′-GCACATCTCCTCCAGGAAGTAGAC-3′ | ||
| Probe | 5′-TCATGCGCTTCTGCCAG BHQ2–3′ | ||
| Giardia tpi | Primary reaction | 54 | |
| AL3543 | 5′-AAATIATGCCTGCTCGTCG-3′ | ||
| AL3546 | 5′-CAAACCTTITCCGCAAACC-3′ | ||
| Secondary reaction | |||
| AL3544 | 5′-CCCTTCATCGGIGGTAACTT-3′ | ||
| AL3545 | 5′-GTGGCCACCACICCCGTGCC-3′ | ||