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. 2018 Jun 7;9(6):689. doi: 10.1038/s41419-018-0731-6

Fig. 3. Effects of GL22 on lipid droplets (LDs), cardiolipin, and C12 metabolism in Huh7.5 cells.

Fig. 3

a, b GL22 treatment for 24 h increased LD content and decreased cardiolipin content in Huh7.5 cells. After incubated with Bodipy 493/503 fluorescent probe and NAO to label LD and cardiolipin, respectively, cells were harvested and measured by flow cytometry. c The metabolism of the fatty acid analog BODIPY C12 was inhibited in Huh7.5 cells treated with GL22 for 3, 6, and 18 h, respectively. d Co-localization of LD and C12 in Huh7.5 cells using confocal laser scanning microscopy after cells were treated without (the upper panel) or with 20 μM GL22 (the lower panel) for 24 h. e Co-localization of cardiolipin and C12 in Huh7.5 cells using confocal laser scanning microscopy after cells were treated without (the upper panel) or with 20 μM GL22 (the lower panel) for 24 h. f TLC resolving Red C12 isolated from Huh7.5 cells treated with 20 μM GL22 for 0, 12, and 24 h, respectively. g Cell viability of Huh7.5 cells treated with 10 μM GL22 in the absence or presence of 0.5 mM ATP or 20 μg/mL cardiolipin, as indicated. CL Cardiolipin