Table 2.
Primers for real-time PCR
| Primers | Sequences | |
|---|---|---|
| E-cadherin | F | 5′-TGCTCACATTTCCCAACTC-3' |
| R | 5′-TCTGTCACCTTCAGCCATC-3' | |
| N-cadherin | F | 5′-CTGACAATGACCCCACAGC-3' |
| R | 5′-TCCTGCTCACCACACTACTT-3' | |
| Vimentin | F | 5′-CTGGATTTCCTCTTCGTGGA-3' |
| R | 5′-CGAAAACACCCTGCAATCTT-3' | |
| GAPDH | F | 5′-GCACCGTCAAGGCTGAGAAC-3' |
| R | 5′-ATGGTGGTGAAGACGCCAGT-3' | |
| Smad3 | F | 5′-GTCTGCAAGATCCCACCAG-3’ |
| R | 5′-AGCCCTGGTTGACCGACT-3’ | |
| miR-16-5p | F | 5′-TAGCAGCACGTAAATATTGGCG-3' |
| R | 5′-TGCGTGTCGTGGAGTC-3’ | |
| U6 | F | 5′-CTCGCTTCGGCAGCACA-3' |
| R | 5′-AACGCTTCACGAATTTGCGT-3' |