Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 7;8:8730. doi: 10.1038/s41598-018-26913-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Conjugation of rGAA with M6PgPs by using copper-free click chemistry. (A) Two different routes to generate M6PgP-conjugated rGAA are schematically represented. In (a) route, rGAA was conjugated with NHS-PEG₄-Azide and the resulting rGAA-azide was linked to DBCO-M6PgP made by M6PgP and DBCO-PEG₄-NHS conjugation. This reaction product was designated as rGAA-azDB-M6PgP. In (b) route, rGAA was conjugated with DBCO-PEG₄-NHS and the resulting rGAA-DBCO was linked to azide-M6PgP, which generated rGAA-DBaz-M6PgP. (B) Sizes of rGAA and its conjugates were analyzed by SDS-PAGE. (C) M6P glycans of rGAA and its conjugates were detected by using Dom9–3xFlag-His₈ blotting analysis: lane 1: PNGase F-treated rGAA, lane 2; rGAA, lane 3; rGAA-azide (a) and rGAA-DBCO (b), lane 4–6; 1:5:20, 1:10:40, and 1:20:80 [rGAA:NHS-PEG₄-Azide:DBCO-M6PgP (a) and rGAA:NHS-PEG₄-DBCO:azide-M6PgP (b)] molar ratio reaction products. (D) Enzyme activities of rGAA-azDB-M6PgP and rGAA-DBaz-M6PgP are represented by white (a) and black (b) bars, respectively. The data represent the averages of three repeated experiments with standard deviations (error bars).