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. 2018 Jun 7;8:8730. doi: 10.1038/s41598-018-26913-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of N-glycans obtained from M6PgP-conjugated rGAA. N-Glycans of rGAA and rGAA-azDB-M6PgP (1:5:20, 1:10:40, and 1:20:80 molar ratio reactions) were analyzed by using HPLC (A). The masses of collected peaks were determined by using MALDI-TOF mass spectrometry (C). The identified glycan peaks were designated by numbers whereas unidentified peaks were represented by*. (B) Relative contents (%) of M6P glycan peaks (7–9) were obtained by the calculation of the integrated peak areas [100× (the area of M6P glycan peaks)/(total areas of all glycan peaks)]. The quantified data represent the averages of three repeated experiments with standard deviations. Symbols are identical to those used in Fig. 1.