Table 1.
List of the glycopeptides identified by HCD and CID MS/MS spectra.
| Peak No | RT (min) | m/z | Charge State | Experimental MW | Identified glycan | MW of glycan | Y0 ion* | Y1 ion* | Calculated MW |
|---|---|---|---|---|---|---|---|---|---|
| 1† | 14.94 | 1392.54 | 2 | 2783.08 | HexNAc(2)Hex(9)# | 1864.45 | 919.60 | 1122.59 | 2783.05 |
| 2 | 17.50 | 788.33 | 2 | 1574.65 | HexNAc(2)Hex(5) | 1216.42 | 359.25 | 562.31 | 1574.67 |
| 3 | 18.67 | 1089.89 | 2 | 2177.78 | HexNAc(2)Hex(7)P | 1620.49 | 558.30 | 761.30 | 2177.79 |
| 4† | 18.89 | 839.82 | 2 | 1677.65 | HexNAc(2)Hex(5) | 1216.42 | 462.22 | 665.28 | 1677.64 |
| 5† | 20.40 | 788.76 | 2 | 1575.52 | HexNAc(2)Hex(5)P | 1296.40 | 280.06 | 483.20 | 1575.46 |
| 6† | 20.62 | 800.28 | 2 | 1598.56 | HexNAc(2)Hex(5)P | 1296.40 | 303.12 | 506.22 | 1598.52 |
| 7 | 20.71 | 850.80 | 2 | 1699.60 | HexNAc(2)Hex(5)P | 1296.40 | 404.21 | 607.19 | 1699.61 |
| 8 | 21.16 | 815.29 | 2 | 1628.57 | HexNAc(2)Hex(5)P | 1296.40 | 333.16 | 536.27 | 1628.56 |
| 9 | 21.84 | 865.81 | 2 | 1729.61 | HexNAc(2)Hex(5)P | 1296.40 | 434.18 | 637.29 | 1729.58 |
| 10† | 22.41 | 1442.04 | 2 | 2882.07 | HexNAc(2)Hex(9)P | 1944.45 | 938.51 | 1141.51 | 2881.96 |
| 11† | 22.74 | 899.33 | 2 | 1796.66 | HexNAc(2)Hex(5) | 1216.42 | 581.24 | 784.31 | 1796.66 |
| 12 | 23.75 | 881.79 | 2 | 1761.58 | HexNAc(2)Hex(5)P | 1296.40 | 466.21 | 669.27 | 1761.61 |
| 13 | 23.97 | 866.34 | 2 | 1730.68 | HexNAc(2)Hex(5) | 1216.42 | 515.24 | 718.29 | 1730.66 |
| 14† | 24.73 | 971.36 | 2 | 1940.72 | HexNAc(2)Hex(5)P | 1296.40 | 645.46 | 848.45 | 1940.86 |
| 15 | 26.96 | 828.31 | 2 | 1654.62 | HexNAc(2)Hex(5)P | 1296.40 | 359.22 | 562.31 | 1654.62 |
| 16 | 27.08 | 1048.85 | 2 | 2095.71 | HexNAc(2)Hex(5)P | 1296.40 | 800.50 | 1003.39 | 2095.90 |
| 17† | 28.38 | 1129.88 | 2 | 2257.76 | HexNAc(2)Hex(6)P | 1458.45 | 800.38 | 1003.46 | 2257.83 |
| 18 | 28.81 | 1052.87 | 2 | 2103.72 | HexNAc(2)Hex(6)P | 1458.45 | 646.23 | 849.38 | 2103.68 |
| 19† | 29.04 | 971.85 | 2 | 1941.70 | HexNAc(2)Hex(5)P | 1296.40 | 646.21 | 849.40 | 1941.61 |
| 20 | 31.33 | 1051.40 | 2 | 2100.76 | HexNAc(2)Hex(5)P | 1296.40 | 805.57 | 1008.52 | 2100.97 |
| 21 | 33.85 | 1028.39 | 2 | 2054.81 | HexNAc(2)Hex(5)P | 1296.40 | 759.47 | 962.48 | 2054.87 |
| 22 | 34.74 | 906.32 | 2 | 1810.64 | HexNAc(2)Hex(5)P | 1296.40 | 515.17 | 718.29 | 1810.57 |
| 23 | 37.15 | 878.83 | 2 | 1755.66 | HexNAc(2)Hex(5)P | 1296.40 | 460.36 | 663.36 | 1755.76 |
*MS/MS spectra delivers information about the sugar structure via glycosidic bond cleavages, and provides molecular mass information for the modified peptide in the form of Y0 (peptide + H+) and Y1 (peptide + HexNAc(1) + H+) ions.
†Their HCD and CID spectra are shown in the Supplementary Figure (Fig. S4).
#Abbreviations: N-acetylhexoamine (HexNAc), Hexose (Hex), Phosphate (P).