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. 2018 Jun 7;8:8720. doi: 10.1038/s41598-018-26978-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Temperature increase induced by laser-treatment of GNRs-FA. B16-BL6 cells were treated with PBS or 50 µg/ml GNRs-FA for 24 hrs. (A) A filter paper, with a well-sized hole for a 96-well format plate, was used to cover the treated cells to guarantee the laser irradiating the target well accurately, and an infrared radiation thermometer was applied to measure the temperature; (B) An 808 nm near infrared laser source was applied for 15 min with different power densities that could be adjusted by the current (0.55A (a), 0.60A (b), 0.65A (c)). The detected temperature (converted to Celsius) was plotted. Results represent 1 of 3 experiments.