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. 2018 Jun 7;9(6):687. doi: 10.1038/s41419-018-0732-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The relative expression of miR-514b-3p and miR-514b-5p was quantified by real-time PCR in CRC cell lines. b, c The overexpression efficiency was confirmed in HCT116 and LOVO cells by real-time PCR. d, e Transwell Matrigel invasion assays were performed in HCT116 and LOVO cells after transfection of miR-514b-3p or miR-514b-5p mimics. f, g Transwell invasion assays were performed in HCT116 and LOVO cells after transfection of miR-514b-3p or miR-514b-5p inhibitors. h. The scratch assays were conducted in LOVO cells after control, miR-514b-3p and miR-514b-5p mimics transfection. i. The morphologic changes were observed under a light microscope in HCT116 and SW480 cells transfected with miR-514b-3p or miR-514b-5p mimics. j, k The effects of miR-514b-3p and miR-514b-5p on MMP2 and MMP9 expression were evaluated using ELISA in LOVO and HCT116 cells. All the experiments above were done for three replicates and repeated at least three times. Error bars represented SEM