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. 2018 Jun 7;9(6):687. doi: 10.1038/s41419-018-0732-5

Fig. 6. The functional role of miR-514b-3p and miR-514b-5p in the EMT progression of CRC cell lines.

Fig. 6

a–d GSEA was performed to identify the difference of gene profiles in control-miRNA group, miR-514b-3p overexpression group and miR-514b-5p overexpression group. e, f Real-time PCR was performed to measure the mRNA expression of EMT markers (E-cadherin, CLDN-1, fibronectin-1 and vimentin) in LOVO and HCT116 after transfection of miR-514b-3p or miR-514b-5p mimics. Error bars represented SEM. g, h Western-blot was conducted to evaluate the protein expression of EMT markers (E-cadherin, CLDN-1, fibronectin-1, and vimentin) in LOVO and HCT116 after transfection of miR-514b-3p or miR-514b-5p mimics. GAPDH was used as an internal control. i Schematic representation for the mechanism of miR-514 regulates EMT in human CRC progression