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. 2018 Jun 7;9(6):694. doi: 10.1038/s41419-018-0723-6

Fig. 2. Histamine suppresses cardiomyocytes autophagy overactivation and cell death in AMI HDC−/− mice.

Fig. 2

a Western blot analysis of the autophagy-related proteins LC3 and p62 in the hearts at 3d post AMI. b Representative electron micrographs (EM) of hearts from HDC−/− mice and WT mice at 3d post AMI. Bar = 1 μm. Quantification of autophagic vacuoles (red arrows) is shown in the right panel (n = 3 for the sham group and n = 5 for each of MI groups). c HDC−/− mice were administered exogenous histamine intraperitoneally for 3 consecutive days at the dose of 1 mg/kg/d or 4 mg/kg/d before exposed to AMI surgery. The levels of LC3 and p62 in hearts were analyzed by immunoblot at 3d post AMI (n = 5 for each group). d HDC−/− mice were treated as c. Representative electron micrographs of hearts at 3d post AMI surgery. Bar = 1 μm. Quantification of autophagic vacuoles (red arrows) is shown in the right panel. n = 3 for the sham group and n = 5 for each of MI groups. e HDC−/− mice were treated as c. Apoptosis was analyzed by TUNEL assay at 3d after surgery. TUNEL-positive nuclei (apoptotic cells) are green. Nuclei stained by DAPI show blue. Cardiomyocytes were labeled with a-actinin. Scale bar, 50 μm. n = 3 for the sham group and n = 5 for each of MI groups. *P < 0.05; **P < 0.01