Table 1.
Primer sequences used for quantitative real-time PCR
| Gene name (Genebank No.) | Primer sequences (5′–3′) | Annealing temperature (°C) |
|---|---|---|
| GAPDH (NM_008084.2) | Forward: TGCACCACCAACTGCTTAG | 55 |
| Reverse: GGATGCAGGGATGATGTTC | ||
| ALP (NM_007431.1) | Forward: GTTGCCAAGCTGGGAAGAACAC | 55 |
| Reverse: CCCACCCCGCTATTCCAAAC | ||
| BSP (NM_008318.3) | Forward: AAAGTGAAGGAAAGCGACGA | 55 |
| Reverse: GTTCCTTCTGCACCTGCTTC | ||
| Col Iα1 (NM_007742.3) | Forward: GAAGGCAACAGTCGATTCACC | 55 |
| Reverse: GACTGTCTTGCCCCAAGTTCC | ||
| OC (NM_001032298.2) | Forward: GAACAGACTCCGGCGCTA | 55 |
| Reverse: AGGGAGGATCAAGTCCCG | ||
| DMP1 (NM_016779.2) | Forward: AGTGAGGAGGACAGCCTGAA | 60 |
| Reverse: GAGGCTCTCGTTGGACTCAC | ||
| OPN (NM_001204203.1) | Forward: TTCACTCCAATCGTCCCTAC | 55 |
| Reverse: TGCCCTTTCCGTTGTTGTC | ||
| TfR1 (NM_011638.4) | Forward: GATCAAGCCAGATCAGCATTCT | 60 |
| Reverse: ACCGGGTGTATGACAATGGTT | ||
| FPN1 (NM_016917.2) | Forward: ACCAAGGCAAGAGATCAAACC | 60 |
| Reverse: AGACACTGCAAAGTGCCACAT | ||
| H-ferritin (NM_010239.2) | Forward: CAAGTGCGCCAGAACTACCA | 60 |
| Reverse: GCCACATCATCTCGGTCAAAA | ||
| L-ferritin (NM_010240.2) | Forward: CCATCTGACCAACCTCCGC | 60 |
| Reverse: CGCTCAAAGAGATACTCGCC |
The specific pairs of primers