Table 2.
Characteristics of the nine transconjugants harboring blaNDM or blaKPC−2.
| Donor species and strain | Origin of donor | Genes | MICs (mg/L) | Other resistance profiles | Plasmid Replicon type (size kb) | Plasmid RFLP profilesa | Donor PFGE patternsb | ||
|---|---|---|---|---|---|---|---|---|---|
| MEM | CTX | NAL | |||||||
| E. coli | |||||||||
| VS2T | Romaine lettuce | blaNDM−5 | 32 | >128 | 4 | AMP, CTF, CAZ, ETP, IPM, STR | X3 (~40) | A1 | A |
| VS1T | Romaine lettuce | blaNDM−5 | >32 | 64 | 4 | AMP, CTF, CAZ, ETP, IPM, STR | X3 (~40) | A3 | A |
| VK70T | Curly endive | blaNDM−5 | >32 | >128 | 4 | AMP, CTF, CAZ, ETP, IPM, STR | X3 (~40) | A4 | C |
| VH1T | Cucumber | blaNDM−5 | >32 | >128 | 4 | AMP, CTF, CAZ, ETP, IPM, STR | X3 (~40) | A1 | B |
| VH3-1T | Cucumber | blaNDM−5 | 32 | 64 | 4 | AMP, CTF, CAZ, ETP, IPM, STR | X3 (~40) | A1 | A |
| C. freundii | |||||||||
| VX9T | Tomato | blaNDM−1, fosA3 | 16 | 128 | 4 | AMP, CTF, CAZ, ETP, IPM, STR FOS | X3 (~40) | A2 | D |
| VK49T | Curly endive | blaNDM−1 | 16 | 64 | 4 | AMP, CTF, CAZ, ETP, IPM, STR, KAN | X3 (~40) | A2 | E |
| K. pneumoniae | |||||||||
| VH1-2T | Cucumber | blaKPC−2 | 16 | 32 | 4 | AMP, CTF, CAZ, ETP, IPM, STR, KAN | F35:A-:B1 (~130) | B | F |
| VH11T | Cucumber | blaKPC−2 | 16 | 16 | 4 | AMP, CTF, CAZ, ETP, IPM, STR, KAN | F35:A-:B1 (~130) | B | F |
| C600 | 0.031 | 0.06 | 4 | STR | |||||
a
RFLP profiles differing by only a few bands (n = 1 ~ 3) were assigned to the same profile.
b
PFGE patterns of the same donor species differing by only a few bands (n = 1 ~5) were assigned to the same group.
AMP, ampicillin; MEM, meropenem; CTX, cefotaxime; CTF, ceftiofur; CAZ, Ceftazidime; ETP, ertapenem; IPM, imipenem; NAL, nalidixic acid; STR, streptomycin; KAN, kanamycin; FOS, fosfomycin.