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. 2018 Jun 1;9:1170. doi: 10.3389/fmicb.2018.01170

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Copyright © 2018 Li, Shen, Yang, Le, Li, Wang, Zhao, Tan, Hu and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LPS profiling, adsorption assay, and spot assay of PA1RG in comparison with PA1 and PA1RG/wzy. (A) LPS profiling. Mutant PA1RG produced LPS lacking long chain O-antigen. Complementary strain PA1RG/wzy displayed the same LPS pattern to that of wild-type PA1. (B) Adsorption assay. Decreased PaP1 attachment was observed for mutant PA1RG, which could be complemented by providing wzy in trans. Data were expressed as mean ± SD from three biological replicates. ns, not significant. ^∗∗∗p < 0.001. (C) Spot assay. Tenfold serial dilutions of PaP1 lysates were applied to the lawns of indicated strains. Phage-resistant PA1RG restored PaP1 susceptibility when bearing the intact gene wzy.