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(A) Strong cytoplasmic staining of glutamine synthetase (magnification 400×). (B) Differential positivity of glutamine synthetase within the same tumor, adjacent liver parenchyma shows positivity in the perivenular hepatocytes (magnification 40×). (C) Diffuse and strong cytoplasmic of Glypican3 staining in the tumor cells. The adjacent liver parenchyma is negative. (magnification 100×). (D) Strong membranous positivity of Glypican3 in more than 10% tumor cells (magnification 400×).
