Table 3.

Patterns of Staining of HSP70, Glutamine Synthetase and Glypican3 in HCC.

Antibody	Pattern of staining	Interpretation (positive/negative)
Glutamine synthetase (GS)	Diffuse strong cytoplasmic positivity unrelated to vascular structures/septae	Positive (19/35)
	Patchy strong positivity along the septae	Negative (11/35)
HSP70	Diffuse strong cytoplasmic with nuclear positivity	Positive (17/35)
	Isolated cytoplasmic positivity	Negative (4/35)
Glypican3 (GPC3)	Cytoplasmic positivity/strong membranous positivity in >10% of cells	Positive (10/35)
	Occasional cells positive/weak cytoplasmic staining	Negative (8/35)