Figure 3.

Protective Effects of Secreted Hutat2:Fc on HIV-1 Tat-Induced Neurotoxicity in Mouse Primary Cortical Neurons

(A) Representative images of mouse primary neurons exposed to HIV-Tat in the presence or absence of KI-M-Hutat2. Cells were stained with MAP-2 (red), TUNEL (green), and a nuclear stain (blue). Scale bar, 100 μm. (B) Comparison of the relative neuronal survival rates of the cells shown in (A) after treatment with each type of conditioned media (n = 3). The calculation method is described in the Materials and Methods. Dunnett’s t test was used for statistical comparisons; **p < 0.01. The results are presented as the means from independent experiments. The error bars denote the SEM. KI-293T-Hutat2, culture supernatant of KI-293T cells; KI-HeLa-Hutat2, culture supernatant of KI-HeLa cells; KI-M-Hutat2, culture supernatant of KI-Ms; KI-U937-Hutat2, culture supernatant of KI-U937 cells.