Figure 3.

Sub-tomogram Averaging Analysis Reveals that Eisosomes with High Membrane Curvature Display a Dense Coat

(A) Cryo-FM (green channel) of section areas over carbon film holes containing Pil1-GFP signals of interest (white circles). Panel V corresponds to Figure 1E.

(B) Virtual slices through electron cryo-tomograms acquired at the corresponding positions.

(C) 2D class averages of eisosome membranes marked by green dashed boxes in (B). In III–VI, the diameter (d) of the outer density layer is indicated by the red dashed circle segment.

(D) 2D class averages of plasma membrane marked by yellow dashed boxes in (B).

(E) Corresponding 3D eisosome shape modeled by segmentation of the plasma membrane.

(F) Cartoon interpretation of coating and membrane curvature in presence of Pil1-GFP.

Scale bars represent 500 nm in (A), 25 nm in (B and E), and 10 nm in (C and D).