Figure 4.

Determination of the Temporal Pattern of Protein Activity after Transposase Delivery by LNPs Compared to Standard Delivery Methods

HeLa cells were transfected with 900 ng of the pPBT/PGK-Puro transposon donor, and subsequently, PB transposase was delivered to the cells by either plasmid DNA transfection (A), transfection of in-vitro-transcribed mRNA (B), or transduction of transposase-loaded LNPs (C) at the indicated time points. Detailed delivery schemes can be seen in the left panels of (A), (B), and (C). Transposase activity was subsequently estimated as described earlier. Triangles, hyPBase; squares, hyPBmut. Data are presented as mean ± SEM and n ≥ 3.