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. 2018 Feb 8;11:170–179. doi: 10.1016/j.omtn.2018.02.001

Figure 3.

Figure 3

miR-106a-5p Inhibits GLIS3 Expression by Targeting its 3′-UTR

(A and B) Immunoblot analysis of chondrocytes transfected with miR-106a-5p (A) or anti-miR-106a-5p (B). The histograms shown under the immunoblot graphs reveal corresponding miR-106a-5p expression levels. (C) miRNA luciferase reporter assay in human chondrocytes cotransfected with wild-type or mutated GLIS3 reporter and miR-106a-5p. The top panel shows the wild-type and mutant forms of the putative miR-106a-5p target sequences in the GLIS3 3′-UTR. Bold and red italicized font indicate the putative miR-106a-5p binding sites within the human GLIS3 3′-UTR. GLIS3 WT, wild-type GLIS3 3′-UTR; GLIS3 Mut, mutated GLIS3 3′UTR. Each bar represents the mean ± SD of at least three independent experiments performed in triplicate. *p < 0.05, **p < 0.01.