Figure 3.

The source of IP-10 on central nervous system. Primary glial cells isolated from suckling mice were cultured in a 37°C incubator containing 5% CO₂. Then, 5 MOI of Japanese encephalitis virus (JEV)-P3 was added to the well, and mRNA samples were collected. (A) IP-10 in the supernatant of glial cells was detected by enzyme-linked immunosorbent assay (ELISA), and (B) the copy number of the JEV-P3 C gene in primary glial cells was measured by quantitative real-time PCR. (C) Brain sections showing co-staining for GFAP/Iba-1 (green) and IP-10 (red) from mice that infected intravenously with 10⁵ plaque-forming units of JEV-P3 or PBS. Nuclei are shown in blue. White arrow represents the colocalization of GAFP and IP-10. Data are shown as the mean ± SEM (n = 3). **p < 0.01; ***p < 0.001.