FIGURE 2.

Oral administration of WOBE437 attenuates acute pain in the hot plate test in BALB/c male mice in a CB1 receptor-dependent manner. (A) Dose-response curve of WOBE437 in the pain latency response in the hot plate test. 50 mg/kg of WOBE437 p.o. was the minimum dose to significantly increase pain threshold. The analgesic effect of (B) 50 mg/kg and (C) 100 mg/kg of WOBE437 was completely abolished by pre-treatment with rimonabant (5 mg/kg, i.p.). All doses are expressed in mg/kg. Rimonabant was injected i.p. 30 min before gavage administration of WOBE437. Data show mean values ± SD of 5–10 mice. Data were compared using Kruskal–Wallis test followed by Mann–Whitney test. ^∗p < 0.05 vs. vehicle; ^#p < 0.05 vs. WOBE437; p.o. per os; ns, no significant.