Figure 10.

Flow cytometry of peripheral blood mononuclear cells (PBMCs) of representative patients. PBMCs (1 × 10⁵) were collected and stained with antibodies against programed death 1 (PD-1), CD8, and CD3. The percpCy5.5-labeled CD3⁺ T cells (middle panel) were gated form the size and granularity dot-plot (left panel), then these CD3⁺ T cells were analyzed by the PE-Cy7 and FITC dot-plot to identify the populations of CD8 and PD-1-expressing cells. Patient 21 had a low PD-1⁺/CD8⁺ ratio in the PBMC cytoblock (A). Patient 41 was with a high PD-1⁺/CD8⁺ ratio in PBMC cytoblock (B).