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. 2018 May 25;9:1138. doi: 10.3389/fimmu.2018.01138

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Copyright © 2018 Buchele, Abendroth, Büttner-Herold, Vogler, Rothamer, Ghimire, Ullrich, Holler, Neurath and Hildner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Colonic donor T cell expansion occurs in an interleukin-23 receptor (IL-23R)- and retinoic acid-related orphan receptor gamma t-independent manner. At day 28–31, colonic lamina propria mononuclear cells of allogeneic hematopoietic stem cell transplantation BALB/c mice with fully established clinical signs of intestinal graft-versus-host disease due to the transfer of allogeneic T cells derived from wild-type (WT) (n = 14) and Il23r^−/− (n = 15) mice (A) or WT (n = 12) and Rorc^−/− (n = 11) mice (B) were analyzed by flow cytometry. The frequency and absolute number of CD4⁺ and CD8⁺ donor T cells are displayed. Data represent pooled results from two independent experiments and were analyzed by Student’s t-test. Data display mean ± SEM.