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. 2018 May 29;9:1164. doi: 10.3389/fmicb.2018.01164

FIGURE 1.

FIGURE 1

Inhibition of influenza virus infection by Lentinula edodes mycelia (LEM) in vitro and in vivo. (A) Cytotoxicity of LEM to cell growth was measured in the presence of 0, 5, 10, 50, 100, 500 μg/ml of LEM. The results are averages from three independent experiments with standard deviations. (B,C) Plaque assays were performed in the presence of 0, 30, 100, and 300 μg/ml of LEM with WSN (B) or SH361 (C). The percentage of the plaque area in the presence of LEM relative to that in the absence of LEM is shown (N > 50). The results are averages from three independent experiments with standard deviations, and the statistical significance was determined by Student’s t-test. (D) Anesthetized mice were intranasally infected with 1,000 pfu of PR8 in the presence (Nasal in red line, n = 8) or absence (Non-treated in black line, n = 8; Oral in blue line, n = 7) of 1 mg/ml LEM. The mouse survival was monitored for 14 days with (Oral in blue line) or without (Non-treated in black line; Nasal in red line) the oral administration of 1 mg/ml LEM. Statistical significance was determined by Gehan–Breslow–Wilcoxon test.